Engineering the Next Generation of IVT Reactors: Reactor Design for Real-Time Monitoring of mRNA Production

Institute

Lehrstuhl für Bioseparation Engineering (TUM-ED)

Type

Bachelor's Thesis / Semester Thesis / Master's Thesis /

Content

experimental / constructive /  

Description

Messenger RNA (mRNA) mRNA has become a key platform for vaccines and future medicines. Its production relies on in vitro transcription (IVT), a sensitive enzymatic reaction that requires stable temperature, efficient mixing, controlled feeding, and reliable sampling.
Real-time monitoring can make IVT processes more efficient and easier to control. However, conventional laboratory reactors are not designed for the direct integration of compact mid-infrared (MIR) systems. A dedicated reactor is therefore needed that combines suitable inlets and outlets with temperature control, mixing, and an MIR measurement.
In this thesis, you will design a compact IVT reactor in CAD, manufacture it by 3D printing, and test it experimentally. You will develop and compare design concepts, integrate feeding, sampling, cooling, and stirring, and evaluate the final prototype with respect to functionality and suitability for IVT and inline MIR monitoring.

Requirements

Enrolled in a Bachelor’s or Master’s program in Biotechnology, Chemical Engineering, Bioprocess Engineering, Mechanical Engineering, Bioengineering, or a related discipline
Interest in reactor design, CAD, additive manufacturing, and bioprocess development
Basic experience with CAD software, 3D printing, or laboratory work is advantageous
Independent, practical, structured, and motivated working style

Possible start

sofort

Contact

Marc Müller
Room: 3427
m.r.muellertum.de

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